Fluorescent cytoplasm and Heinz bodies of hemoglobin Köln erythrocytes: evidence for intracellular heme catabolism.
نویسندگان
چکیده
Hb Köln, one of the common mutant hemoglobins responsible for unstable hemoglobin disease, was found to be degraded to a fluorescent yellow pigment (FYP) in circulating erythrocytes. FYP is responsible for a strong green fluorescence observed in the cytoplasm and is particularly abundant in the Heinz bodies of Köln RBC. Front face fluorometry and fluorescence microscopy showed that Heinz bodies emit 10% to 20% of the fluorescence of RBCs. Hb-free FYP was obtained by means of a cellulose column separation of the cytoplasm or from a precipitate formed during the incubation of Köln RBC cytoplasm at 50 degrees C. The absorption and emission spectra of FYP are consistent with those of dipyrroles.
منابع مشابه
Fluorescent Cytoplasm and Heinz Bodies of Hemoglobin K#{246}ln Erythrocytes: Evidence for Intracellular Heme Catabolism
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ورودعنوان ژورنال:
- Blood
دوره 65 4 شماره
صفحات -
تاریخ انتشار 1985